The event of revolutionary antibacterial supplies is essential for addressing wounds contaminated with bacterial biofilms. Superior nanomaterials that allow non-antibiotic antibacterial methods provide new potentialities for treating bacterial infections by eliminating pathogens with out counting on antibiotics. Herein, we introduce non-toxic and biocompatible DNA–copper cluster nanosheets (DNS/CuNCs) as efficient antibacterial brokers. DNS/CuNCs can cut back bacterial floor motility and the secretion of virulence components by interfering with quorum sensing, and thereby inhibit biofilm formation and improve their potential as prophylactic antibacterial brokers. Notably, DNS/CuNCs exhibit important in vitro bactericidal exercise in opposition to Staphylococcus aureus and Pseudomonas aeruginosa and disrupt established floor biofilms within the presence of hydrogen peroxide (H2O2). That is attributed to the synergistic results of their bodily ultrathin properties and peroxidase-like exercise, which result in a rise in intracellular ROS ranges in micro organism, thereby attaining antibacterial and biofilm-disrupting results. In vivo, DNS/CuNCs successfully eradicate bacterial infections, promote wound therapeutic, and restore regular tissue morphology with out toxicity to mammalian cells. With their mixed skills to inhibit biofilm formation, exhibit antibacterial exercise, and disrupt biofilms, together with glorious biocompatibility, DNA-templated CuNCs emerge as extremely promising candidates for preventive and scientific antibacterial therapies.
