Exact management over floor properties is essential for the design of nanocarriers in biomedical purposes. These properties affect organic interactions. Useful co-monomers can be utilized to tailor the floor chemistry of nanocarriers synthesized in radical heterophase polymerization in aqueous part. Nonetheless, attaining related management over nanocarriers derived from pure supplies in inverse miniemulsion, akin to protein nanocapsules, stays difficult. Right here, we reveal how the floor practical group density of protein nanocapsules will be tuned systematically by various the hydrophobicity of the continual part in the course of the synthesis by way of the press response between hydrophilic azide-modified proteins and a hydrophobic dialkyne crosslinker. By adjusting the solvent combination of toluene and cyclohexane, the interfacial properties of the droplets are modified, influencing the partial denaturation of the protein and orientation of the amine-terminated lysine residues. This, in flip, impacts the accessibility of the azide teams for the crosslinking. Adjustments in solvent composition moreover affect the solubility and reactivity of the crosslinker, thereby modulating the diploma of azide functionalization. This enables for exact management over the variety of unreacted azide teams accessible for subsequent biorthogonal click on reactions. We reveal that the multifunctional floor, with amine, azide and alkyne teams, permits the simultaneous attachment of various molecules to the nanocapsule. Lastly, we present that whereas adjustments in steady part hydrophobicity lead solely to minor adjustments in protein corona composition, they considerably have an effect on macrophage uptake, doubtless as a result of variations in floor amine density. Our mixed findings present a novel method for tailoring the floor performance of nanocapsules, facilitating extra exact and versatile biofunctionalization methods, significantly for focused drug supply.
